mona_PHX said:They are not contradictory, they measure different things, and this is the single most common misreading of a certificate.
Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in the refrigerator.
Day 1: 100.7% purity
Day 14: 99.6% purity
Day 28: 98.1% purity
Minimal degradation over 28 days when properly refrigerated. This confirms the standard 28-day beyond-use date is appropriate. Beyond 28 days, I'd reconstitute a fresh vial to be safe.
A narrower follow-up, since the general answer is now clear:
Why two laboratories report different purity for the same batch, and which number to believe?
dave_SLC said:Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in…
Vendor quality matters when discussing certificates of analysis. I've tested my last 6 batches through Janoshik and purity has ranged from 98.7% to 100.7%. Consistency is key.
For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.
Sigma-Aldrich — Research-Grade Standards
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Shop Reference StandardsClosing the loop on my own question.
Purity and content being different measurements was the piece I was missing. The vial was overfilled and pure, which is a very different problem from the one I thought I had.
anna.melb_AU said:Vendor quality matters when discussing certificates of analysis.
Agreed, and mass spec answers a question purity does not: identity. High purity tells you one thing dominates the chromatogram, not that it is the molecule you ordered. A mass within about a dalton of expected is the identity check.