PurityPaulOR said:Inter-laboratory variation comes from real methodological differences, not sloppiness: which reference standard was used and how it was itself…
I am more sceptical of certificates as a category than this thread is. The document tells you about a sample somebody chose to send, at a moment in time, and nothing about the vial in your hand. Independent purchase-and-test is a different quality of evidence and everything else is inference.
ingrid_STO said:I have a certificate reporting 99.2% purity and 6.1mg content on a vial sold as 5mg, and I cannot work out whether those two numbers are consistent or…
Vendor quality matters when discussing certificates of analysis. I've tested my last 3 batches through Janoshik and purity has ranged from 97.8% to 99.0%. Consistency is key.
For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.
labquiet_amy said:I am more sceptical of certificates as a category than this thread is.
HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean:
- Purity %: Should be >97%. Below 95% is concerning.
- Retention time: Should match reference standard for the peptide
- Related substances: Degradation products should be <2% total
- Single impurity: No individual impurity should exceed 1%
My last batch: 99.7% purity, 0.1% total related substances. Clean chromatogram with single sharp peak at expected retention time. Exactly what you want to see.
Sigma-Aldrich — Research-Grade Standards
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Shop Reference StandardsOne thing that is still open after NeuroNate’s answer:
Whether a purity figure and a content figure can disagree without one of them being wrong?
Closing the loop on my own question.
Purity and content being different measurements was the piece I was missing. The vial was overfilled and pure, which is a very different problem from the one I thought I had.